Benjamin Groff, Author at Instituto Milenio BASE

As part of its participation in the 61st Antarctic Scientific Expedition (ECA61) of INACH, the team from the UC Molecular Biodiversity Laboratory, led by Dr. Juliana Vianna, crossed the Antarctic Circle to enhance the surveillance and monitoring of Highly Pathogenic Avian Influenza (H5N1) in the frozen continent.

Punta Arenas.—Under strict biosecurity measures and in coordination with the Chilean Antarctic Institute (INACH), the Chilean Navy, Army, and Air Force, scientists Dr. Fabiola León, Claudia Ulloa, and Carolina Márquez embarked on their scientific journey aboard the Betanzos vessel, operated by DAP. Their mission is to collect samples and conduct laboratory analyses as part of their work at the UC Molecular Biodiversity Laboratory, led by Dr. Juliana Vianna (BASE/CRG, Lili, UC Institute for Sustainable Development), in collaboration with the Millennium Institute BASE.

“After successfully detecting positive cases of Highly Pathogenic Avian Influenza (H5N1) in Antarctica during the ECA60 expedition of INACH in January 2024, we have been working diligently to analyze samples and coordinate research teams studying birds such as penguins, cormorants, and skuas in Antarctica,” says Dr. Juliana Vianna, a Ph.D. in Biological Sciences with a specialization in Ecology and a faculty member at UC.

The Antarctic scientist, who also serves as the deputy director of the Millennium Institute CRG, highlights the importance of studying wildlife health in Antarctica due to the connectivity between species and the continent’s crucial role in global ecosystem balance. “The migration and movement of species can influence the spread of avian influenza, potentially increasing mortality rates. This underscores the importance of continuing field studies to monitor affected areas and species,” emphasizes Dr. Vianna.

The scientific work with birds in Antarctica aims to strengthen the surveillance and monitoring of Highly Pathogenic Avian Influenza (H5N1).

Throughout 2023-2024, the collection and analysis of samples in Antarctica were coordinated by researchers Dr. Fabiola León (UC/BASE/CRG, Lili) and Eduardo Pizarro (UC/BASE/CRG), with international support from the vessel Le Commandant Charcot, operated by Ponant Cruises. In 2025, the research is being conducted aboard the Betanzos vessel, part of the DAP group, reaching beyond the Antarctic Circle into the so-called “deep Antarctica,” specifically in Lagotellerie Island and Aves Island in Margarita Bay.

“Our field experience has been essential in ensuring safe and rigorous scientific work under strict biosecurity measures. It is important to highlight that our campaigns cover a wide geographic range and are conducted at different times of the season, allowing for surveillance over a timeline that helps determine the status of avian influenza at various stages of the nesting season for both flying and non-flying birds,” says Dr. Fabiola León. Her research has taken her to sample emperor, Adélie, gentoo, and king penguins in locations such as Snow Hill Island, Danger Islands, Red Rock Ridge, Cape Hallett, and Sims Island.

Essential biosecurity measures

The “Prevention and Control Protocol for Highly Pathogenic Avian Influenza (HPAI) in Antarctica,” issued by the Chilean Antarctic Institute (INACH), aligns with international recommendations from the Scientific Committee on Antarctic Research (SCAR). These biosecurity measures apply to all expeditions taking place from January to March 2025 in Antarctica and follow the “One Health” guidelines for preserving the health of the unique and fragile Antarctic ecosystem.

“The study of avian influenza and its evolution is essential for understanding viral dynamics and global public health. At our Millennium Institute BASE, we are committed to scientific excellence, working rigorously and collaboratively to address this constantly evolving phenomenon. High-quality science not only allows us to obtain accurate results but also provides the necessary tools to anticipate and mitigate risks associated with the spread of infectious diseases,” states Dr. Elie Poulin, director of the Millennium Institute for Antarctic and Sub-Antarctic Ecosystem Biodiversity.

As part of these protective efforts, Personal Protective Equipment (PPE) plays a crucial role. As indicated by the Chilean Institute of Public Health (ISP), PPE includes specialized gear, apparatus, or devices designed to protect the body—either partially or entirely—from specific risks. These measures help prevent occupational diseases, reduce accidents, and minimize their impacts.

Researchers Fabiola León, Claudia Ulloa, and Carolina Márquez wear biosecurity suits for sample collection in Antarctica.

The Millennium Institute BASE/UC/CRG research team emphasizes that in Antarctica, the use of masks, gloves, and coveralls protects both people and the living organisms inhabiting the region. “Introducing new pathogens from humans to birds and mammals or unintentionally transporting and spreading them from healthy colonies to other locations is a critical concern that requires extreme caution,” they note. Additionally, pre- and post-sampling cleaning of equipment before entering any area is a mandatory part of the daily protocol during field expeditions.

“Highly pathogenic avian influenza is a major concern within the international scientific community,” states Dr. Ricardo Jaña, head of INACH’s Scientific Department. “A special group has been organized to gather and analyze evidence across different Antarctic research sites, establishing an information system through reports,” he adds.

Jaña highlights that this effort extends beyond Chile, as other countries are also contributing data from their research expeditions. “South Korea has recently reported findings in the Fildes Peninsula area, and Chile, through its campaign in Margarita Bay, has also documented cases from different bird populations,” he notes.

INACH’s Scientific Department head and expert in Natural Resources/Glaciology affirms that “this concern extends beyond Antarctica.” “In the local region, a working group led by the Ministry of Agriculture has been established, with our colleague Marcelo González serving as an institutional representative,” he concludes.

Through tools such as infographics and specialized publications, the Chilean Antarctic Institute has provided guidelines and recommendations, which can be downloaded from https://www.inach.cl/expedicion-antartica/protocolo-influenza-aviar/.

Blood Sample Analysis in Penguins Helps Study Highly Pathogenic Avian Influenza (H5N1)

The early detection and epidemiological surveillance of highly pathogenic avian influenza (HPAI) in the Antarctic Peninsula are being led by various virology and wildlife teams from the Chilean Antarctic Institute’s National Antarctic Science Program (PROCIEN). Since 2024, two avian influenza testing laboratories have been operating at Escudero Base and O’Higgins Base to support areas of national and international interest and establish connections with the Scientific Committee on Antarctic Research (SCAR) programs.

This initiative is complemented by Antarctic governance actions, where Chile is leading an intersessional contact group under the Antarctic Treaty on Highly Pathogenic Avian Influenza. This group includes, for example, Dr. Luis Valentín Ferrada, a professor at the University of Chile and researcher at the Millennium Institute BASE.

A joint effort between universities, top-tier research institutes, and Antarctic operators underscores the significance of collaborative work and scientific research in Antarctica to understand and mitigate the risks associated with avian diseases in this unique region.

By: Nadia Politis
Photographs: Constanza Barrientos/Millennium Institute BASE

Aboard the French research vessel Le Soléal (Ponant Cruises) collaborative effort between PhD. Claudio González-Wevar (UACh/BASE/IDEAL) and Professor Hamish G. Spencer (University of Otago) combines expertise in molecular genomics, evolutionary biology, and biogeography to investigate the distribution, diversity, and evolutionary history of molluscs in the Southern Ocean’s unique and understudied ecosystems. The expedition aims to advance the understanding of subantarctic molluscs through detailed collection and study.

Marking their tenth expedition, the BASE Millennium Institute and Ponant Cruises continue groundbreaking research, this time on Macquarie, Stewart and Auckland Islands, led by PhD. González-Wevar, head of the Laboratory of Antarctic and Subantarctic Molecular Genomics and Ecology (LAGEMAS-UACh). The project is also strengthened by the expertise of Professor Hamish G. Spencer, Sesquicentennial Distinguished Professor from the Department of Zoology at the University of Otago, New Zealand. Together, the team focuses on sampling and analyzing intertidal ecosystems, highlighting the importance of these islands, which host key study groups such as mollusc species Nacella (e.g., terroris, macquariensis), Laevilitorina (e.g., mcphersonae, venusta), and Margarella (e.g., violacea, macquariensis), among others.

The scientific work involves sampling from the subantarctic intertidal zone. Image: Hamish Spencer

“The participation of PhD. Hamish Spencer from the University of Otago has been invaluable. His expertise enabled us to access Stewart Island and collect specimens for our studies”, González-Wevar adds. The expedition covers rare and remote locations, including Traills Bay, the Auckland Islands in New Zealand, and Macquarie Island in Australia. These areas offer unparalleled opportunities for understanding biodiversity and ecological dynamics.

Professor Hamish G. Spencer, underscores the significance of the research: “The biogeography of the Southern Ocean is fascinating and yet understudied. Nevertheless, we have long understood that the world’s strongest oceanic current, the Antarctic Circumpolar Current (ACC), is the major physical player in the distribution of organisms in the subantarctic. In a long-standing collaboration with Professor Claudio González-Wevar of BASE, I am interested in understanding how long-distance dispersal in the ACC has shaped the marine biodiversity around the shores of the Southern Ocean. We have used molluscs as our model organisms and have previously collaborated on projects about limpets (Nacella and Siphonaria), topshells (Margarella), and periwinkles (Laevilacunaria and Laevilitorina)”.

Spencer elaborates on the mission’s goals: “We now want to expand our work to include more populations and species from the New Zealand and Australian subantarctic islands (Auckland, Campbell and Macquarie). These islands are especially important because they have acted as refugia for some species during past Ice Ages. We are aiming on this cruise to collect samples of all of the above genera, as well as some others for new projects (e.g., the bivalve Gaimardia). Our preliminary work on some of these groups suggests that there has been significant evolutionary radiation in this region, even though it is connected by long-distance dispersal to Patagonia, the Antarctic Peninsula, and the subantarctic islands in the Atlantic and Indian sectors”.

The Auckland and Campbell Islands (New Zealand) and Macquarie Island (Australia) are key sites for scientific research.

A Partnership Driving Scientific Excellence

Since 2022, the collaboration between the BASE Millennium Institute and Ponant Cruises has paved the way for groundbreaking research. “This partnership allows us to conduct more and better science while strengthening ties with world-class research teams”, says PhD. Elie Poulin, director of the BASE Millennium Institute.

PhD. Claudio González-Wevar and Phd. Hamish Spencer embarking on a subantarctic expedition.
Image: C. González-Wevar

Together with Dr. Spencer, González-Wevar delves into population genetics, phenotypic plasticity, phylogenetics, and the unique characteristics of New Zealand molluscs. The Auckland, Campbell, and Macquarie Islands are pivotal to their work, particularly Macquarie Island, which UNESCO declared a World Heritage site in 1997. This island’s geological significance, as the only location where mantle rocks are exposed above sea level, underscores its importance for scientific inquiry.

Professor Spencer concludes: “The ultimate goal of our work is to explain the origin of intertidal and nearshore Southern Ocean biodiversity, especially the range of evolutionary outcomes, from widespread species to island-group endemics”.

UNESCO highlights New Zealand’s subantarctic islands as biodiversity hotspots where Antarctic and subtropical waters converge, fostering high marine productivity and unique ecosystems. “These islands boast significant endemism, especially among birds, plants, and invertebrates”, the organization points. This biodiversity underscores the global significance of research conducted by González-Wevar and his team.

By: Nadia Politis, BASE Millennium Institute

Main picture: French vessel Le Soléal, operated by Ponant Cruises. By Hamish Spencer.